Publication Date

1-1-1992

Document Type

Dissertation/Thesis

First Advisor

Vary, Patricia S.

Degree Name

B.S. (Bachelor of Science)

Legacy Department

Department of Biological Sciences

Abstract

The construction of a stable cloning vector would be very useful to the study of genetics of Bacillus megaterium. It seems reasonable to assume that a vector made with a natural resident plasmid of this species will be stable. This is the rationale behind cloning the 5.4-kilobase plasmid of B. megaterium. The 5.4-kb plasmid and the vector were each cut with a restriction endonuclease that produced blunt ends. These two plasmids were ligated using the enzyme T4 DNA ligase. The recombinant plasmid was transformed into E. coli. Transformant DNA was then analyzed using restriction enzvmes. Because of errors in the restriction analysis data used, only part of the 5.4-kb plasmid has been cloned. However, this hybrid plasmid may still be useful in constructing cloning vectors for use in B. megaterium. and other bacilli.

Comments

Includes bibliographical references.

Extent

16 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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