Publication Date

5-4-2017

Document Type

Dissertation/Thesis

First Advisor

Ryzhov, Victor

Degree Name

B.S. (Bachelor of Science)

Legacy Department

Department of Chemistry and Biochemistry

Abstract

With various antibiotics becoming illegitimate due to bacteria building up resistance to them, it becomes imperative to find new antibiotics to replace the ones that no longer work. In theory, this can be accomplished by inhibiting the IspF enzyme which can be found within the Methylerythritol phosphate (MEP) pathway. Four different cell cultures, one treated and containing no IPTG, one treated and containing IPTG, a control with IPTG, and a control lacking IPTG were found to have grown successfully at a steady pace over a period of 5 hours. This means that the overexpression of IspF within the cell cultures does not affect their ability to grow at a steady rate. An overexpression of IspF resulted in a large peak pertaining to CMP, whereas an unaltered sample lacked a defined peak. This should be useful in future work because it will be easier to see if potential inhibitors are successful.

Extent

7 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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