Sims, Thomas L.
B.S. (Bachelor of Science)
Department of Biological Sciences
Self-incompatibility is a cell-cell recognition system in higher plants that is based on the ability of the pistil to distinguish non-self pollen from self pollen. In Petunia hybrida, selfincompatibility is controlled by a single locus, the S-locus. The sequences governing S-RNase expression are not known and an understanding of this would enable us to better manipulate selfincompatibility response in flowers. This study attempted to correlate the degree of reporter gene activity with varying amount of 5’ flanking sequence present on different gene constructs. Reporter gene activity was assayed by fluorometric measurements of GUS ((3-glucuronidase) enzyme activity normalized to total soluble protein. Pistils were collected from transgenic plants with different gene constructs and protein extracts prepared. Extracts were used for protein and enzyme assays. Of the 34 plants assayed, only 5 showed GUS expression above background. Because of this low number of plants expressing GUS, we were unable to draw any conclusion about the effect of 5’ flanking sequences on S-RNase expression.
Bose, Madhulika, "Regulation of S-Rnase gene expression in petunia hybrida" (1997). Honors Capstones. 953.
Northern Illinois University
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