Publication Date
1-1-2011
Document Type
Dissertation/Thesis
First Advisor
Stafstrom, Joel P.
Degree Name
B.S. (Bachelor of Science)
Legacy Department
Department of Biological Sciences
Abstract
Purpose and Methodology: The purpose of this study was to determine the role of DRG and GFP in response to heat stress in Arabidopsis by examining whether any DRG mutant combination inhibits the formation of heat stress granules (HSGs) following exposure of plants to heat stress. Methods utilized in the experimentation include the polymerase chain reaction (PCR) and gel electrophoresis to screen for plants containing the desired combination of genes, a root-growth assay to study the relationship between phenotype and genetic composition, and confocal microscopy to observe the formation of HSGs in root tips. Findings: The genetic composition of DRG genes and GFPs does have an effect on the phenotype of Arabidopsis. Conversely, the presence of a wild type DRG gene fused to GFP (DRGI-GFP or DRG2-GFP) does not complement the non-functional DRG genes.
Recommended Citation
McKinney, Lindsey, "Isolation and analysis of Arabidopsis thaliana lines bearing DRG knock-out mutations and overexpressing GFP protein fusions: Are wild-type DRGs necessary for heat stress granule formation?" (2011). Honors Capstones. 705.
https://huskiecommons.lib.niu.edu/studentengagement-honorscapstones/705
Extent
16 pages
Language
eng
Publisher
Northern Illinois University
Rights Statement
In Copyright
Rights Statement 2
NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.
Media Type
Text
