Publication Date

1-1-2014

Document Type

Dissertation/Thesis

First Advisor

Meganathan, Rangaswamy

Degree Name

B.S. (Bachelor of Science)

Legacy Department

Department of Biological Sciences

Abstract

It is well established that for aerobic growth E.coli requires either Menaquinone (MK), Ubiquinone (Q) or 2-octaprenyl-3-methyl-6- methoxy-1,4-benzoquinone (MMQ) as electron carrier to grow on glycerol minimal medium (GMM). Therefore, a double mutant strain blocked in both the MK and Q pathways shouldn’t grow on GMM. The following double mutant strains were tested for growth on GMM (A ubiE Amen A, AubiH Amen A, AubiF Amen A, AubiB AmenA and AyigP AmenA). Surprisingly, it was found that the AubiB AmenA mutant grew after a long lag and AubiF AmenA mutant grew as expected due to the Q pathway intermediate MMQ serving as electron carrier. In GMM AubiB AmenA double mutant showed an increase in the rate of growth and growth yield. It is a known fact that all the Q pathway gene mutants including ubiB cannot grow on succinate minimal medium (SMM). The ubiB mutant has been previously shown to accumulate small amount of Q in stationary phase. To our surprise, the double mutant was also able to grow on succinate and lactate minimal media once adapted to grow in GMM. Quinone analysis of the AubiB AmenA mutant using HPLC revealed that it made significant amount of Q. Therefore, the reason behind the growth of double mutant strain is due to increase in the amount of Q.

Comments

Includes bibliographical references.

Extent

10 unnumbered pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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