Publication Date

Fall 12-10-2024

Document Type

Student Project

First Advisor

Grayburn, Scott W.

Degree Name

B.S. (Bachelor of Science)

Department

Department of Biological Sciences

Abstract

This study investigates the potential of bacterial metalloprotease genes as therapeutic agents for Alzheimer's Disease (AD). The research focuses on Pseudomonas 20ei1 (P. 20ei1), a bacterial species isolated from lagoon water, which contains pitrilysin genes that share similarities with human insulysin. Six pqqL-related genes were identified in P. 20ei1 for this study. The research methodology involved bioinformatic studies, bacterial cell culture in various media, RNA extraction, purification, and cDNA synthesis. Quantitative PCR analysis was performed to analyze gene expression levels in different cDNA samples. The bacteria grown in the medium with insoluble phosphate has shown higher expression of selected genes consistently. BLAST searches were conducted to compare P. 20ei1 genes with the human genome and other bacterial species. The study aims to identify and characterize new enzymes capable of degrading amyloid-beta (Aβ) peptides, which are implicated in AD pathology. By exploring microbial Aβ-degrading enzymes, particularly pitrilysin and related proteins, the research seeks to uncover new insights into Aβ degradation mechanisms and potentially develop novel therapeutic approaches for Alzheimer's disease. The findings of this study could contribute to more effective AD treatment strategies.

Suggested Citation

Uday K. Chigurupati, 2024

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