Publication Date

2024

Document Type

Student Project

First Advisor

Singh, Pallavi

Department

Department of Biological Sciences

Abstract

Salmonella is a widespread pathogen that can cause gastrointestinal tract infections in humans. Infections in humans can occur through the increased consumption of bison. Bison intestines can be reservoirs, therefore intestinal microbiome may be an important factor in Salmonella colonization. This project aims to determine the prevalence of Salmonella from different managements and unravel potential risks associated with pathogen spread. Bison fecal samples were collected seasonally from four sites for two years. These samples were incubated in R-10 broth for 48 hours followed by TTB broth for another 48 hours at 37⁰C. The broth was then spread onto BG and SS agar plates for selective Salmonella growth. Presumptive Salmonella colonies were confirmed through polymerase chain reaction using target gene-specific primers. Future studies will evaluate management practices, seasonal variation, and comprehensive analysis of the bison intestinal microbiome. The results from this project will provide information to develop improved food safety measures.

Publisher

Northern Illinois University

Share

COinS