Swingley, Wesley D.
M.S. (Master of Science)
Department of Biological Sciences
This project characterizes two independent, previously undescribed microbial dark matter organisms from the candidate phyla JL-ETNP-Z39 and NBK19. These characterizations are based on seven single amplified genomes (SAGs) for JL-ETNP-Z39 and six SAGs for NBK19. All SAGs used were provided by the Bigelow Laboratory for Ocean Sciences Single-Cell Genomics Center (scgc.bigelow.org). Bioinformatic analyses using a combination of publicly available software and custom scripting written in the computer programming languages Python and R were carried out to determine the basic genomic properties, evolutionary relatedness, representative environmental pangenomes, and metabolic properties of these organisms. Analyses indicated that the examined JL-ETNP-Z39 SAGs are likely Gram-negative, flagellated, aerobes that are members of a single species belonging to a larger candidate phylum, while examined NBK19 SAGs are Gram-negative aerobes belonging to two or more branches of a novel phylum.
Nuccio, Daniel Adam, "Bioinformatic Anlayses of Identified But Uncultivated Microbial Dark Matter Organisms Using Archival Single Amplified Genomes (sags)" (2020). Graduate Research Theses & Dissertations. 7500.
Northern Illinois University
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