Publication Date


Document Type


First Advisor

Swingley, Wesley D.

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Biological Sciences


This project characterizes two independent, previously undescribed microbial dark matter organisms from the candidate phyla JL-ETNP-Z39 and NBK19. These characterizations are based on seven single amplified genomes (SAGs) for JL-ETNP-Z39 and six SAGs for NBK19. All SAGs used were provided by the Bigelow Laboratory for Ocean Sciences Single-Cell Genomics Center ( Bioinformatic analyses using a combination of publicly available software and custom scripting written in the computer programming languages Python and R were carried out to determine the basic genomic properties, evolutionary relatedness, representative environmental pangenomes, and metabolic properties of these organisms. Analyses indicated that the examined JL-ETNP-Z39 SAGs are likely Gram-negative, flagellated, aerobes that are members of a single species belonging to a larger candidate phylum, while examined NBK19 SAGs are Gram-negative aerobes belonging to two or more branches of a novel phylum.


74 pages




Northern Illinois University

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