Publication Date
2020
Document Type
Dissertation/Thesis
First Advisor
Swingley, Wesley D.
Degree Name
M.S. (Master of Science)
Legacy Department
Department of Biological Sciences
Abstract
This project characterizes two independent, previously undescribed microbial dark matter organisms from the candidate phyla JL-ETNP-Z39 and NBK19. These characterizations are based on seven single amplified genomes (SAGs) for JL-ETNP-Z39 and six SAGs for NBK19. All SAGs used were provided by the Bigelow Laboratory for Ocean Sciences Single-Cell Genomics Center (scgc.bigelow.org). Bioinformatic analyses using a combination of publicly available software and custom scripting written in the computer programming languages Python and R were carried out to determine the basic genomic properties, evolutionary relatedness, representative environmental pangenomes, and metabolic properties of these organisms. Analyses indicated that the examined JL-ETNP-Z39 SAGs are likely Gram-negative, flagellated, aerobes that are members of a single species belonging to a larger candidate phylum, while examined NBK19 SAGs are Gram-negative aerobes belonging to two or more branches of a novel phylum.
Recommended Citation
Nuccio, Daniel Adam, "Bioinformatic Anlayses of Identified But Uncultivated Microbial Dark Matter Organisms Using Archival Single Amplified Genomes (sags)" (2020). Graduate Research Theses & Dissertations. 7500.
https://huskiecommons.lib.niu.edu/allgraduate-thesesdissertations/7500
Extent
74 pages
Language
eng
Publisher
Northern Illinois University
Rights Statement
In Copyright
Rights Statement 2
NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.
Media Type
Text
