Faridah Yusof

Publication Date


Document Type


First Advisor

Jones, Claude R.

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Chemistry


CIS-dichlorodiammineplatinum(II); DNA; Aspergillus oryzae


The reaction of S₁ nuclease from Aspergillus oryzae with DNA was investigated and the reaction was found to be more complex than previously thought. Besides rapidly hydrolyzing single stranded DNA S₁ nuclease also showed monoesterase activity towards the 5'-mononucleotides (the product of S₁ nuclease hydrolysis of single stranded DNA) and also exonuclease activity towards double stranded DNA. These two activities complicate the standard addition assay used to find the denaturation content in bulk samples of DNA. Cis-dichlorodiammineplatinum(II) (cis-Pt(NH₃)₂Cl₂) is an anti-tumor agent currently used in the chemotherapy. It has been reported that the reaction of cis-Pt(NH₃)₂Cl₂ with DNA causes denaturation of the DNA. The standard addition assay was used to investigate this point. It was found that binding of the platinum complex to DNA could take place in two ways, clustered and random, and different forms of the platinum complex were shown to react with DNA in different manners. The reaction of cis-Pt(NH₃)₂Cl₂ with DNA gave a predominantly clustered type binding whereas the reaction of cis-Pt(NH₂)₂(H₂O)₂ with DNA gave random binding. This was shown by dialysis experiments, denaturation values determined by standard addition assay, differences in the annealing ability after the platinum removal from the DNA and the hydroxyapatite separations.


Bibliography: pages 57-59.


viii, 59 pages




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