Parth R. Rao

Publication Date

2015

Document Type

Dissertation/Thesis

First Advisor

Bujarski, Jozef J.

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Biological Sciences

LCSH

Microbiology; Virology; RNA viruses--Research; Bromoviridae--Genetics--Research; Genetic recombination--Research; Plant protoplasts--Research; Barley; Virus diseases--Epidemiology--Research

Abstract

The study was performed to map the homologous recombination crossover regions between the genomic RNA3 of Fescue strain and Russian strain of Brome Mosaic Virus in barley protoplasts. Homologous recombination crossover regions between the genomic RNA 2 of both strains were analyzed previously in high selection pressure system using three different co-infected host plants. Thus, protoplast system was used to lower the selection pressure by eliminating the systemic infection phase. Both strains were introduced in different concentration to observe if the parental strain dominance can be lowered in the final clones. However, fescue strain showed dominance over Russian strain in two out of three ratios in BMV RNA3 co-inoculation. There were 12 crossover regions generated within RNA3, and most of them were consistent in all three ratios. Intercistronic region and PE regions were obvious hotspots observed in recombinants of all three ratios. Co-inoculation of RNA2 in protoplasts confirmed the hotspots mapped in the previous study in whole plant system. Also, analysis of RNA2 clones in protoplasts resulted in similar recombination frequencies to the whole plant systems experiment regardless of the selection pressure, which shows true mosaic nature of BMV.

Comments

Advisors: Jozef Bujarski.||Committee members: Mitrick Johns; Thomas Sims.

Extent

86 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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