Publication Date

1988

Document Type

Dissertation/Thesis

First Advisor

Briles, W. Elwood

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Biological Sciences

LCSH

Erythrocytes; Leucocytes; Immune serums

Abstract

Alloantisera against erythrocyte alloantigens of the chicken were evaluated for reactivity to leukocyte antigens of the same species. Leukocytes and erythrocytes were tested by indirect immunofluorescence (IIF) for reactivity with previously produced alloantisera against antigens of the A, C, and K systems. Separate suspensions of leukocytes and erythrocytes from individual chickens of appropriate genotypes were treated with Al, A4, C4, and K2 alloantisera followed by goat anti-chicken IgG Fc conjugated with fluorescein isothiocyanate (FITC). Observation of slide mounts of such treated cells under the fluorescent microscope revealed that C4 and K2 antigens were present on leukocytes. The results using the Al and A4 antisera were inconclusive.

Comments

Bibliography: pages [53]-56.

Extent

vi, 56 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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