Publication Date

1993

Document Type

Dissertation/Thesis

First Advisor

Polans, Neil O.

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Biological Sciences

LCSH

Peas--Genetics; Genomes; Gene mapping

Abstract

I have constructed a linkage map of the pea genome based upon classical and molecular-marker traits. Existing morphological, isozyme and RFLP (restriction fragment length polymorphism) markers were supplemented with a powerful new approach called RAPDs, or Randomly Amplified Polymorphic DNAs. The RAPD procedure, developed by Williams et al. (1990) and Welsh and McClelland (1990), involves the amplification of random DNA segments by the arbitrarily primed-polymerase chain reaction (AP-PCR) using single primers of arbitrary nucleotide sequence. Decamer primers consisting of 50%-80% GC content have generated between one and nine DNA products across a range of pea accessions, revealing polymorphism in thirty-nine percent of the samples tested. In each case, the presence of a product was scored as the dominant allele state and its absence in a contrasting individual as the recessive allele state. Over ninety percent of the polymorphisms observed segregated in Mendelian fashion among the F2 progeny of a selected cross in a generally consistent and reproducible manner. A total of 107 polymorphisms (that segregated in Mendelian fashion) were generated. About half of these markers were assigned to positions on an integrated pea nuclear genomic map by linkage association with previously mapped genetic loci.

Comments

Includes bibliographical references (pages [70]-71)

Extent

79 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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