Publication Date

1979

Document Type

Dissertation/Thesis

First Advisor

Frampton, Elon W.

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Biological Sciences

LCSH

Anacystis nidulans; Plasmids; DNA

Abstract

Plasmid deoxyribonucleic acid (DNA) was isolated from Anacystis nidulans cells labeled with [³H]deoxyadenosine by preparative equilibrium density gradient centrifugation using ethidium bromide-cesium chloride gradients. Neutral sucrose velocity gradient centrifugation (5-20%) showed two major peaks of radioactivity with sedimentation coefficients of 24 and 48S as calculated by a computer program. This material was shown to be covalently closed circular (CCC) DNA by conversion to open circular (OC) material with decreased S values by ionizing radiation. Molecular weights of the CCC DNA molecules were determined from the S values to be 4.5 x 10⁶ daltons and 22.9 x 10⁶ daltons. Guanine + cytosine content was determined by analytical cesium chloride equilibrium density gradient centrifugation to be 60.2 and 53.06 moles % for the two plasmids. The plasmids were resistant to elimination in cultures treated with a variety of curing agents.

Comments

Includes bibliographical references.||Includes illustrations.

Extent

vi, 53 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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