Starzyk, Marvin J.
M.S. (Master of Science)
Department of Biological Sciences
Thermus aquaticus YT-1; Thermophilic bacteria; Plasmids
Thermus aquaticus YT-1 is an extreme bacterial thermophile containing four plasmids of 5.7, 8.9, 10.3, and 11.4 megadaltons. Elimination of the 5.7, 8.9, and 11.4 megadalton plasmids was accomplished by growth in the presence of novobiocin. Higher rates of curing were obtained at suboptimal growth temperatures than at the optimal growth temperature normally associated with the organism. Wild type T. aquaticus YT-1 and plasmid cured strains were characterized with respect to antibiotic sensitivities, sensitivities to a variety of metal salts, and several metabolic functions in an attempt to ascribe phenotypic traits encoded by the various plasmids. No differences were detected between the plasmid cured strains and the wild-type Thermus aquaticus YT-1.
Becker, Deborah A., "Plasmid curing in Thermus aquaticus YT-1" (1986). Graduate Research Theses & Dissertations. 4413.
Northern Illinois University
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