Publication Date

1986

Document Type

Dissertation/Thesis

First Advisor

Starzyk, Marvin J.

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Biological Sciences

LCSH

Thermus aquaticus YT-1; Thermophilic bacteria; Plasmids

Abstract

Thermus aquaticus YT-1 is an extreme bacterial thermophile containing four plasmids of 5.7, 8.9, 10.3, and 11.4 megadaltons. Elimination of the 5.7, 8.9, and 11.4 megadalton plasmids was accomplished by growth in the presence of novobiocin. Higher rates of curing were obtained at suboptimal growth temperatures than at the optimal growth temperature normally associated with the organism. Wild type T. aquaticus YT-1 and plasmid cured strains were characterized with respect to antibiotic sensitivities, sensitivities to a variety of metal salts, and several metabolic functions in an attempt to ascribe phenotypic traits encoded by the various plasmids. No differences were detected between the plasmid cured strains and the wild-type Thermus aquaticus YT-1.

Comments

Bibliography: pages [69]-77.

Extent

77 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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