Publication Date

1981

Document Type

Dissertation/Thesis

First Advisor

Hampel, Arnold E.

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Biological Sciences

LCSH

Hamsters; Ovaries; Cells--Morphology

Abstract

Cell morphology profiles of several partial revertants of the temperature-sensitive Chinese Hamster Ovary cell line mutant, tsHl, which exhibits a defect in its leucyl-tRNA synthetase enzyme, were examined using light and scanning electron microscopy and were compared to the morphology profiles observed in wild-type and mutant cultures. Wild-type and tsHl morphology differences have been previously documented. Although the morphology of these cells changes as they progress through the cell cycle, wild-type cultures typically appear epithelial throughout interphase and display a randan distribution pattern, as opposed to tsHl cultures which tend to display a bipolar, spindle-shaped morphology. The relationship between these observed morphological changes and the altered leucyl-tRNA synthetase enzyme in idle mutant cells is unknown; however, morphological characterization of tsHl revertants suggests an apparent link between the enzyme defect and the observed bipolar morphology, since recovery of enzyme activity in the revertants is accompanied by a corresponding decrease in the appearance of the bipolar morphology type and an increase in the more spread out epithelial shape characteristic of wild-type cultures.

Comments

Includes bibliographical references.||Includes illustrations.

Extent

viii, 105 pages, 7 unnumbered pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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