Publication Date

1983

Document Type

Dissertation/Thesis

First Advisor

Vanderkooi, Garret

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Chemistry

LCSH

Anesthetics--Physiological effects; Synaptosomes; Neural transmission

Abstract

The acetylcholinesterase, (NaK)-ATPase and (Mg)- ATPase activities of rat brain synaptosomal membranes were measured as a function of increasing concentrations of local anesthetics. It was found that both the ouabain- sensitive (NaK)-ATPase and the ouabain-insensitive (Mg)- ATPase required about the same concentrations of local anesthetic for 50% inhibition, viz., lidocaine, 44 mM; tetracaine, 6 to 10 mM; dibucaine, 1.8 to 3.5 mM; and chlorpromazine, 0.22 to 0.25 mM. The logarithms of these concentratiohs are proportional to the logarithms of the respective partition coefficients. Inhibition of acetylcholinesterase required the following concentrations of anesthetic for 50% inhibition: procaine, 0.86 mM; lidocaine, 7.0 mM; tetracaine, 0.2 mM; dibucaine, 0.65 mM; and chlorpromazine, 0.21 mM. Inhibition of acetylcholinesterase by tetracaine and dibucaine was demonstrated to be competitive with substrate.

Comments

Bibliography: pages 67-70

Extent

ix, 79 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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