Publication Date

1969

Document Type

Dissertation/Thesis

First Advisor

Mittler, Sidney||Abdel-Hameed, Fathi, 1938-

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Biological Sciences

LCSH

Drosophila melanogaster; Variation (Biology)

Abstract

Male Drosophila melanogaster inbred Oregon R flies were given various injections of distilled water and inorganic salts and irradiated with 1600r of x-rays. These treated males were then mated in a 1:1 ratio with the same strain of three day old virgin females daily for twelve days. A measurement of the dominant lethality for each brood day was determined by the hatchability of the eggs laid by the mated females. The various treatments are distilled water, 0.85 mg/ml sodium chloride, 2.50 mg/ml sodium chloride and 1.612 mg/ml calcium chloride which is of equal molarity with the 0.85 mg/ml sodium chloride. These males were injected prior to irradiation. The treatments and the control produced the most dominant lethals during brood days six through nine, that represent the spermatocyte state at the time of irradiation. Fewer dominant lethals were produced during brood days one through five (spermatozoa and spermatids). The fewest dominant lethals were produced during brood days ten through twelve which is during the stage of spermatogonia. This is in concurrence with Schmid's, (1961), work.Male Drosophila melanogaster inbred Oregon R flies were given various injections of distilled water and inorganic salts and irradiated with 1600r of x-rays. These treated males were then mated in a 1:1 ratio with the same strain of three day old virgin females daily for twelve days. A measurement of the dominant lethality for each brood day was determined by the hatchability of the eggs laid by the mated females. The various treatments are distilled water, 0.85 mg/ml sodium chloride, 2.50 mg/ml sodium chloride and 1.612 mg/ml calcium chloride which is of equal molarity with the 0.85 mg/ml sodium chloride. These males were injected prior to irradiation. The treatments and the control produced the most dominant lethals during brood days six through nine, that represent the spermatocyte state at the time of irradiation. Fewer dominant lethals were produced during brood days one through five (spermatozoa and spermatids). The fewest dominant lethals were produced during brood days ten through twelve which is during the stage of spermatogonia. This is in concurrence with Schmid's, (1961), work. It has been shown by this experiment that distilled water, 0.85 mg/ml sodium chloride, 2.50 mg/ml sodium chloride and 1.612 mg/ml calcium chloride injections do protect against radiation damage in many of the brood days when tested against an uninjected control. The results obtained from the distilled water treatment suggests that the effect of oxygen free radicals described in the literature is not obtained.

Comments

Includes bibliographical references.||Includes illustrations.

Extent

65 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

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