Publication Date

1993

Document Type

Dissertation/Thesis

First Advisor

Hampel, Arnold E.

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Biological Sciences

LCSH

Catalytic RNA; HIV (Viruses)--Gene therapy; Genetic transformation; Genetic engineering

Abstract

The hairpin ribozyme has been shown to cleave several heterologous RNA targets. In this thesis two hairpin ribozymes were shown in vitro to cleave two separate RNA sequences that are expressed by the virus in vivo. In vitro characterization was performed on these ribozymes to provide kinetic data as well as to optimize the target length. One of the HIV-l sequences is found at position #451 to #465. This targeted region is called TAR and is required for regulation of transcription of all the HIV-1 genes. The other sequence is found at position #6009 to #6024. This region codes for two separate genes called TAT and REV. TAT is a protein that associates with the TAR region to increase the rate of transcription of viral genes. REV is also a protein that associates with a region called the REV responsive element. This association is needed for proper splicing of the viral genes to occur.

Comments

Includes bibliographical references (pages [52]-55)

Extent

vi, 55 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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