Publication Date

1987

Document Type

Dissertation/Thesis

First Advisor

Meechan, Paul

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Biological Sciences

LCSH

Animal genetics; Ultraviolet radiation--Physiological effect; Ultraviolet radiation--Genetic aspects

Abstract

Ultraviolet light-induced effects on biological systems are well documented. The most intensely studied effects are lethality, mutagenesis, and inhibition of DNA synthesis. It is generally agreed that the basis of these effects lie in the response to DNA lesions such as the K5-6) cyclobutane dimer, which is the most prominent lesion formed after irradiation with 254 nm UV. There are, however, a variety of minor lesions that, though not formed as efficently as the (5-6) dimer, may actually be responsible for the induced effects. A number of mechanisms have evolved that enable a cell to remove or tolerate the various chemical modifications induced in DNA by UV radiation. Common to prokaryotes and eukaryotes are excision repair and photoreactivation. Postreplication repair is also common to both groups, although the specific repair mechanisms are fundamentally different. E. coli has an inducible damage tolerance system termed the SOS response that has no counterpart in eukaryotic cells. The research project described here details the construction of a cosmid library of the chicken genome. Evidence is given that the library was completed and is representative of the entire genome. Two methods for isolating the chick photolyase gene are discussed, and a method for generating a PR+ mammalian cell line is suggested. Finally, experiments that will delineate (5-6) effects from effects due to minor lesions in mammalian cells via use of the chick photolyase are discussed.

Comments

Bibliography: pages [62]-73.

Extent

73 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

Download

Share

COinS