Publication Date

1995

Document Type

Dissertation/Thesis

First Advisor

Johns, Mitrick A.

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Biological Sciences

LCSH

Genetics; Experimental; Cloning

Abstract

A mouse MyoD cDNA probe was used to screen a Clonetec lambda phage porcine genomic library. Three distinct clones were isolated. Restriction fragments containing the genic regions were isolated from the clone that showed the strongest hybridization. These fragments were ligated into the vector plasmid pBS+. Further subcloning of these regions resulted in several smaller plasmids that were subjected to sequencing. The resultant data showed a high degree of homology with sheep, mouse, and human MyoD sequences. Sequencing has shown that Exon I has been subcloned along with nearly a kilobase of sequence at the 5’ end.

Comments

Includes bibliographical references (pages [40]-43).

Extent

43 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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