Duane Heaton

Publication Date

1976

Document Type

Dissertation/Thesis

First Advisor

Frampton, Elon W.

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Biological Sciences

LCSH

Plasmids; Anacystis nidulans; DNA

Abstract

Plasmid deoxyribonucleic acid (DNA) was isolated from cells labeled with [³H] uracil or [³H]deoxyadenosine by preparative equilibrium density gradient centrifugation using ethidium bromide-cesium chloride gradients. Subsequent velocity sedimentations on neutral sucrose gradients (5-20%) disclosed a major peak of radioactivity with a sedimentation constant of 54 to 57S as calculated by comparison with an internal standard and by a computer program, respectively. Exposure of the plasmid DNA to ionizing radiation before centrifugation converted it to a lower S value consistent with the conversion of covalently closed circular DNA (CCC DNA) to open circular DNA. Based on the S constants for the CCC DNA, the molecular weight of the plasmid is in the range of 30 to 34 x 10⁶ daltons, end there are about 4 copies per genome. A variety of treatments did not eliminate the plasmid and its possible function remains undetermined.

Comments

Includes bibliographical references.

Extent

vi, 55 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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