Publication Date

1983

Document Type

Dissertation/Thesis

First Advisor

Frampton, Elon W.

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Biological Sciences

LCSH

Bacteriophages; Electrophoresis; Escherichia coli

Abstract

CsCl isopycnic density gradient centrifugation of lysates of UV- induced cultures of Escherichia coli strain 15 derivatives E. coli 15T- (thy) and E. coli 15 TAU (thy, arg, ura), yielded three phage peaks with buoyant densities of 1.509 g/cm³ (coliphage CP15A), 1.482 g/cm³ (CP15B), and 1.464 g/cm³ (CP15C). Agarose gel electrophoresis of EcoRI, HindiII, and BamHI endonuclease digests of the DNA from these three defective phage types yielded different digestion patterns, implying a unique genome for each phage type. Molecular weight estimates of genome size by agarose gel electrophoresis were 28 x 10⁶ daltons for CP15A, 26 x 10⁶ daltons for CP15B, and 25 x 10⁶ daltons for CP15C. Although the genomes are different, SDS-polyacrylamide ael electrophoresis revealed that the structural proteins of the tailed phages CP15B and CP15C were similar, whereas the CP15A proteins produced an entirely different pattern. The 65.8 x 10⁶ dalton Pl-like plasmid reported by Ikeda et al. (1970) was observed in agarose gels of two E. coli 15 derivatives.

Comments

Bibliography: pages 44-47

Extent

vi, 47 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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