Document Type



Chromatofocusing of soybean (Glycine max L.) leaf lipoxygenases revealed three distinct peaks of activity. Based on their isoelectric points (pis), pH optima, and mutant analysis it appears that the leaf isozymes are different from those described from mature soybean seed. At least one leaf lipoxygenase appears to differ from those found in hypocotyls. The pis of the main bands of the three leaf lipoxygenase peaks are 6.67, 5.91, and 5.67. The pH optima curves of three active fractions exhibit peaks at pH 6.2, 5.5, and 8.5, respectively. One of the fractions has two polypeptides with slightly different molecular weights, both of which react to soybean seed lipoxygenase antibodies. The other two fractions contain a polypeptide of unit molecular weight reacting with the lipoxygenase antibodies.

Publication Date


Original Citation

Grayburn, W.S., R. Schneider, T.R. Hamilton-Kemp, G. Bookjans, K. Ali, D.F. Hildebrand. (1991) Soybean leaves contain multiple lipoxygenases. Plant Physiol. 95: 1214-1218.


Department of Biological Sciences

Legacy Department

Department of Biological Sciences






American Society of Plant Biologists



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