Document Type
Article
Abstract
Chromatofocusing of soybean (Glycine max L.) leaf lipoxygenases revealed three distinct peaks of activity. Based on their isoelectric points (pis), pH optima, and mutant analysis it appears that the leaf isozymes are different from those described from mature soybean seed. At least one leaf lipoxygenase appears to differ from those found in hypocotyls. The pis of the main bands of the three leaf lipoxygenase peaks are 6.67, 5.91, and 5.67. The pH optima curves of three active fractions exhibit peaks at pH 6.2, 5.5, and 8.5, respectively. One of the fractions has two polypeptides with slightly different molecular weights, both of which react to soybean seed lipoxygenase antibodies. The other two fractions contain a polypeptide of unit molecular weight reacting with the lipoxygenase antibodies.
Publication Date
1-1-1991
Recommended Citation
Grayburn, W.S., R. Schneider, T.R. Hamilton-Kemp, G. Bookjans, K. Ali, D.F. Hildebrand. (1991) Soybean leaves contain multiple lipoxygenases. Plant Physiol. 95: 1214-1218.
Original Citation
Grayburn, W.S., R. Schneider, T.R. Hamilton-Kemp, G. Bookjans, K. Ali, D.F. Hildebrand. (1991) Soybean leaves contain multiple lipoxygenases. Plant Physiol. 95: 1214-1218.
Department
Department of Biological Sciences
Legacy Department
Department of Biological Sciences
ISSN
0032-0889
Language
eng
Publisher
American Society of Plant Biologists
