Author

Ryan Ulaszek

Publication Date

1-1-1998

Document Type

Dissertation/Thesis

First Advisor

Conway, Sonya

Degree Name

B.S. (Bachelor of Science)

Department

Department of Biological Sciences

Abstract

This semester I have been involved in ligation of L3, a gene in the La France virus, into the PgemT vector system as well as transfecting Ecoli with our ligated vector. This entire process involves creating a cDNA copy of the L3 RNA viral gene, amplification of the cDNA utilizing PCR, ligating it in to PgemT and then transforming the bacteria. These are the experimental procedures that I followed.

Extent

18 pages, 7 unnumbered pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Alt Title

PCR, its development and application to my research

Media Type

Text

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