Johns, Mitrick A.
B.S. (Bachelor of Science)
Department of Biological Sciences
This lab manual will give students an introduction to some basic laboratory procedures used in PCR and cloning. Standard lab protocols often assume the technician already knows certain basic lab techniques. This manual will prepare students to follow these protocols and start designing experiments on their own. The theory behind PCR and cloning is stressed in this manual, because a clear understanding of the processes involved is essential for experimentation. It allows the student to pin-point any flaws in a procedure, leave out any unnecessary steps, and begin to develop their own methods. These are all signs of a thinking technician. Practice protocols with explanations of terminology and techniques are included in the manual as well as a trouble shooting guide.
Mason, Katherine, "PCR and cloning : a lab manual" (1997). Honors Capstones. 868.
Northern Illinois University
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