Author

Ashley Mottar

Publication Date

1-1-2011

Document Type

Dissertation/Thesis

First Advisor

Bode, Barrie P.

Degree Name

B.S. (Bachelor of Science)

Department

Department of Biological Sciences

Abstract

Cultured cancer cell lines are a widely used approach in our biology research. Our lab uses several human liver cancer lines to study the role of two amino acid transporters (ASCT2 and LAT1) during growth. To validate the identity of each line for comparative results, a means of positive ID is needed. Short tandem repeat (STR) analysis is a method that uses the polymerase chain reaction (PCR) to examine 'w' specific DNA regions, called loci, which surround specific genes. Originally used in forensic analysis and paternity testing, STR analysis has recently become incorporated into research laboratories. The reason is due to the estimate that up to one third of published research is thought to be erroneous due to misidentification of cell lines used in biomedical studies. To proactively address this problem, I have embarked on an effort to establish DNA fingerprints for the human cell lines used in my lab's cancer research. The study involves the isolation of genomic DNA from human liver cancer cell lines and subsequent identification of the cell line via STR analysis. The hypothesis is that each cell line investigated will yield a unique DNA fingerprint. Genomic DNA was isolated, processed, and submitted to an STR analysis core facility for data acquisition. The data presented will reflect my conclusions drawn

Comments

Includes bibliographical references.

Extent

23 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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