B.S. (Bachelor of Science)
Department of Biological Sciences
The objective of this research is to characterize the genetic differentiation of several midwestern geographic populations of Ascaris suum. and to understand if this genetic variation is due to gene flow resulting from the transport of pigs or if, instead, isolated "island" populations undergo independent differentiation. The method of data acquisition was Random Amplified Polymorphic DNA (RAPD) fingerprinting. With this method, an oligonucleotide primer is used to amplify regions of genomic DNA; the amplified products are separated by agarose gel electrophoresis and visualized by staining. The polymorphisms were scored and analyzed using the Hardy-Weinberg equation and F statistics. The data indicates that there is great genetic differentiation and therefore little gene flow among the seven A. suum populations. Further research with the use of isozyme and RFLP techniques and larger population sizes will allow for more complete analysis of the genetic variability.
Lindquist, Rachel L., "Estimates of genetic variation among Midwestern populations of Ascaris suum using RAPD fingerprinting" (1993). Honors Capstones. 491.
7 pages, 5 unnumbered pages
Northern Illinois University
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