B.S. (Bachelor of Science)
Department of Biological Sciences
Sperm-egg ‘binding assays have been developed as diagnostic tests to determine fertility in humans, horses, and cattle. The aim of this study was to develop a similar assay for use in porcine species. Porcine oocytes were obtained from slaughterhouse ovaries. The oocytes were dried on to microscope slides, where they could be stored for up to three months. Whole semen was obtained the day after ejaculation, diluted, and co-incubated with the oocytes on the slide for 10 minutes at 37C. The sperm-egg complexes were rinsed and stained with an ultraviolet DNA stain. The number of sperm bound per oocyte was counted and recorded. As conclusive farrow rate data becomes available, the binding data will be analyzed to determine if there is a statistically significant correlation between binding and overall fertility in the porcine species.
Jessee, Cari A., "Development of a sperm binding assay for boar semen" (1996). Honors Capstones. 401.
Northern Illinois University
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