Publication Date

1-1-1996

Document Type

Dissertation/Thesis

First Advisor

Didion, Brad

Degree Name

B.S. (Bachelor of Science)

Department

Department of Biological Sciences

Abstract

Sperm-egg ‘binding assays have been developed as diagnostic tests to determine fertility in humans, horses, and cattle. The aim of this study was to develop a similar assay for use in porcine species. Porcine oocytes were obtained from slaughterhouse ovaries. The oocytes were dried on to microscope slides, where they could be stored for up to three months. Whole semen was obtained the day after ejaculation, diluted, and co-incubated with the oocytes on the slide for 10 minutes at 37C. The sperm-egg complexes were rinsed and stained with an ultraviolet DNA stain. The number of sperm bound per oocyte was counted and recorded. As conclusive farrow rate data becomes available, the binding data will be analyzed to determine if there is a statistically significant correlation between binding and overall fertility in the porcine species.

Comments

Includes bibliographical references.

Extent

7 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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