Publication Date

1-1-2009

Document Type

Dissertation/Thesis

First Advisor

Johnson-Wint, Barbara

Degree Name

B.S. (Bachelor of Science)

Department

Department of Biological Sciences

Abstract

In preparation to quantify beta-actin and alpha-smooth muscle actin content in loaded and unloaded native rat tail tendon organ culture models, several methodologies were developed in the present paper. Since alpha-smooth muscle actin has not been monitored in tendon in vitro, developing the methods to culture and quantitatively detect alpha-smooth muscle actin, the protein marker for fibroblast to myofibroblast transition were developed. In addition, quantifying the amount of alpha-smooth muscle actin mRNA, using reverse transcription polymerase chain reaction (RT-PCR) assays was investigated and novel oligonucleotide primers were developed. A method to identify four highly conserved actin isoforms; beta cytoplasmic, gamma cytoplasmic, alpha- smooth muscle and gamma smooth muscle actin, in a tissue environment was created.

Comments

Includes bibliographical references.

Extent

23 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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