Yasui, Linda S.
B.S. (Bachelor of Science)
Department of Biological Sciences
Glioblastoma multiforme (GBM) is a deadly brain cancer that currently does not have a definite cure. Studies on specific targets of treatment have been conducted with the intentions of decreasing malignancy and recurrence. Cancer stem cells (CSCs) are suspected to be a vital factor in the recurrence of tumors. The role of DNA-dependent protein kinase (DNA-PK), an enzyme with a significant role in repairing DNA damage, and its effect on autophagy was studied in GBM cells containing DNA-PK, GBM cells lacking DNA-PK, and CSCs. Each type of cell line had a group of cells with radiation treatment and a group without treatment. Analysis of autophagosomes in transmission electron microscope (TEM) images demonstrated the rate of autophagy in response to radiation and the absence of DNA-PK. The absence of DNA-PK was intended to provide a better understanding of the metabolic pathways that are taken when cancer cells are exposed to radiation. By studying how autophagy occurs in the differentiated GBM cells and cancer stem cells, improvements can be made in the radiation treatment against cancer.
Latgnotha, Anita, "The Role of DNA-PK in Autophagy" (2019). Honors Capstones. 1271.
Northern Illinois University
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