Publication Date

1-1-2016

Document Type

Dissertation/Thesis

First Advisor

Bujarski, Jozef J.

Degree Name

B.S. (Bachelor of Science)

Department

Department of Biological Sciences

Abstract

Brome mosaic virus (BMV) is a positive-sense, single-stranded RNA virus that infects plants throughout the world, including barley, tobacco, and wheat. Although this virus is not known for its ability to cause widespread crop damage, researchers frequently utilize BMV to understand more about other RNA viruses, such as human immunodeficiency virus. RNA2 of BMV’s tripartite genome contains a sequence that is thought to be responsible for regulating recombination of RNA3. Currently, there are no studies investigating this function in the Nicotiana benthamiana plant model. Thus, determining how the RNA-dependent RNA polymerase of RNA2 influences recombination in this model will increase understanding of this important mechanism of viral fitness and evolution. A mutation (DR7) was induced in RNA2 and cloned into Agrobacterium tumefaciens. Following agro-infiltration into N. benthamiana, total RNA was extracted and RT-PCR was used to amplify BMV RNA3. Although viral RNA could not be amplified, this project demonstrated the importance of plant age in the infection cycle of BMV.

Schnizlein Senior Thesis Final Draft.pdf (607 kB)
Schnizlein Senior Thesis Final Draft.pdf

Schnizlein Senior Thesis Final Draft.docx (178 kB)
Schnizlein Senior Thesis Final Draft.docx

Extent

10 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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