Hagen, Timothy J.
B.S. (Bachelor of Science)
Department of Chemistry and Biochemistry
Antibiotic resistance is one of the greatest public health challenges of our time. There is an urgent need to discover new antibiotics to combat these drug resistant infections. The MEP pathway is essential to most bacteria and not found in humans which makes it a desirable target for novel antibiotic discovery. The IspF enzyme is the fifth enzyme in the MEP pathway. Screening of a library of approximately 3000 compounds by the Hagen group identified several sulfa drugs that were ligands for the IspF enzyme. The purpose of this research was to synthesize sulfonamide derivative compounds for IspF inhibition. In this project, the target sulfonamide compounds have fluorescent properties. A fluorescent probe will allow the development of a quantitative ligand binding assays for the IspF enzyme. In this work, a fragment merging technique was utilized to design target molecules that should bind to the IspF enzyme. The target molecules were successfully synthesized; however, the purity was below acceptable guidelines. Several methods were attempted to purify the compounds.
Sayles, Amber L., "Synthesis of a,?-dithienylpolyenes as potential model compounds for polythiophene-polyacetylene copolymers" (2018). Honors Capstones. 1080.
Northern Illinois University
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