Publication Date

12-2-2016

Document Type

Dissertation/Thesis

First Advisor

Hosmane, Narayan S., 1948-

Degree Name

B.S. (Bachelor of Science)

Department

Department of Chemistry and Biochemistry

Abstract

Boron Neutron Capture Therapy (BNCT) is a bimodal cancer treatment that involves killing cancer cells through a nuclear reaction of two nontoxic species, boron-10 (10B) and thermal neutrons. There are currently only two FDA-approved drugs for this promising area of cancer therapy, so present research focuses on the synthesis of novel compounds with high boron content as well as methods for their delivery into the tumor cell. A fluorescent tag can allow the observation of the delivery vehicle’s uptake during biodistribution studies. Accordingly, fluorescein was conjugated with iodinated 1-methyl-o-carborane and characterized using Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance spectroscopy (NMR), elemental analysis, and mass spectrometry (MS). The biological potential of the fluorescein-tagged 1-methyl-o-carborane conjugate was then evaluated using flow cytometry, cytotoxicity, and biological distribution studies with squamous cell carcinoma (SCC-VII) and pancreatic cancer (MIA PaCa-2) cell lines using phase-contrast, fluorescent, and confocal microscopy.

Extent

16 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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