Hosmane, Narayan S., 1948-
B.S. (Bachelor of Science)
Department of Chemistry and Biochemistry
Boron Neutron Capture Therapy (BNCT) is a bimodal cancer treatment that involves killing cancer cells through a nuclear reaction of two nontoxic species, boron-10 (10B) and thermal neutrons. There are currently only two FDA-approved drugs for this promising area of cancer therapy, so present research focuses on the synthesis of novel compounds with high boron content as well as methods for their delivery into the tumor cell. A fluorescent tag can allow the observation of the delivery vehicle’s uptake during biodistribution studies. Accordingly, fluorescein was conjugated with iodinated 1-methyl-o-carborane and characterized using Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance spectroscopy (NMR), elemental analysis, and mass spectrometry (MS). The biological potential of the fluorescein-tagged 1-methyl-o-carborane conjugate was then evaluated using flow cytometry, cytotoxicity, and biological distribution studies with squamous cell carcinoma (SCC-VII) and pancreatic cancer (MIA PaCa-2) cell lines using phase-contrast, fluorescent, and confocal microscopy.
Kuzmanic, Lucas A., "Synthesis and Biological Evaluation of Fluorescein-Tagged Carborane for Boron Neutron Capture Therapy (BNCT)" (2016). Honors Capstones. 1077.
Northern Illinois University
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