Title

Design of Turn-On Near-Infrared Fluorescent Probes for Highly Sensitive and Selective Monitoring of Biopolymers

Publication Title

Angewandte Chemie - International Edition

ISSN

14337851

E-ISSN

15213773

Document Type

Article

Abstract

Simple, sensitive, and selective detection of specific biopolymers is critical in a broad range of biomedical and technological areas. We present a design of turn-on near-infrared (NIR) fluorescent probes with intrinsically high signal-to-background ratio. The fluorescent signal generation mechanism is based on the aggregation/de-aggregation of phthalocyanine chromophores controlled by selective binding of small-molecule “anchor” groups to a specific binding site of a target biopolymer. As a proof-of-concept, we demonstrate a design of a sensor for EGFR tyrosine kinase—an important target in cancer research. The universality of the fluorescent signal generation mechanism, as well as the dependence of the response selectivity on the choice of the small-molecule “anchor” group, make it possible to use this approach to design reliable turn-on NIR fluorescent sensors for detecting specific protein targets present in the low-nanomolar concentration range.

First Page

8440

Last Page

8444

Publication Date

5-25-2020

DOI

10.1002/anie.202000108

PubMed ID

32135034

Keywords

aggregation, biosensors, EGFR tyrosine kinase, fluorescent probes, phthalocyanines

Department

Department of Chemistry and Biochemistry

COinS