Publication Date


Document Type


First Advisor

Bujarski, Jozef

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Biological Sciences


Brome mosaic virus (BMV) is a positive sense, single-stranded RNA virus that infects a wide array of plant hosts globally including: wheat, oats, maize, barley, and rye. Since its discovery in the 1940s, BMV has served as a model organism in uncovering the modalities of viral infectivity, composition, translation, and replication. In many viruses, it is typically imperative to recruit cellular machinery to produce viral proteins as their coding capacity is limited. Therefore, there are sequences and structures within the leader sequences allow them to be competitive with the eukaryotic mRNAs. Although BMV has been thoroughly researched in these other various aspects of its lifecycle, the 5’-unstranslated region (UTR) of BMV RNA3 has not been characterized extensively in relation to the translation of 3a; otherwise known as the viral movement protein (MP). This project focuses on programming an in vitro wheat germ system with synthetic mutants containing progressive deletions as means of uncovering sequence and structure-specific requirements for production of MP encoded in the first open reading frame (ORF) of RNA3.


78 pages




Northern Illinois University

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