Publication Date


Document Type


First Advisor

Hagen, Timothy J.

Degree Name

Ph.D. (Doctor of Philosophy)

Legacy Department

Department of Chemistry and Biochemistry


Flavonoids; Urease--Inhibitors; Antimalarials; Aminopeptidases--Inhibitors; Organic chemistry; Biochemistry


This dissertation explores the inhibition of prominent enzymatic pathways important for both medicinal and agricultural applications. The research involved three distinct projects: First, an enzymatic urease inhibitory assay was optimized for plate-based screening of tree bark extracts to compare with ex vivo results from simulated-barn floor manure slurry assays performed by Dr. Wayne Zeller (USDA, Madison, WI). It wafs discovered that the measured ammonia abatement activities of each extract exhibited a correlation in both the enzymatic and ex vivo assays. Second, aminoalkylated quercetin analogs were synthesized and screened for antimalarial activity. The most potent species were found to exhibit sub-micromolar inhibitory values against multidrug-resistant strains of Plasmodium falciparum, the malarial parasite. Finally, a library of potential inhibitors of methionine aminopeptidase, an enzyme necessary for bacterial proliferation, were synthesized or purchased and screened for enzymatic inhibitory activity. A number of sub-micromolar inhibitors were discovered, and the most potent compounds were screened in a host-cell viability assay by Dr. Jonathon Audia (USA, Mobile, AL).


Advisors: Timothy J. Hagen.||Committee members: Melvin R. Duvall; James R. Horn; Douglas A. Klumpp; Victor V. Ryzhov.


389 pages




Northern Illinois University

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