Publication Date

2015

Document Type

Dissertation/Thesis

First Advisor

Horn, James R.

Degree Name

Ph.D. (Doctor of Philosophy)

Department

Department of Chemistry and Biochemistry

LCSH

Biochemistry||Biophysics||Molecular biology||Antigen-antibody reactions||Thermodynamics||Immunoglobulins||Chemistry, Analytic--Technique

Abstract

Antibody fragments are attractive alternatives to conventional antibodies for both therapeutic and diagnostic applications. One example, single-domain VHH antibodies, which are derived from camelid heavy chain-only antibodies, display comparable binding affinity to conventional antibodies despite the significantly reduced binding interface. Here, an anti-RNase A VHH single-domain antibody and its antigen, RNase A, are used as a model antibody-antigen system for the three studies described in this dissertation.

Comments

Advisors: James R. Horn.||Committee members: Gary Baker; Timothy Hagen; Victor Ryzhov; Yanbin Yin.

Extent

165 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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