Horn, James R.
Ph.D. (Doctor of Philosophy)
Department of Chemistry and Biochemistry
Biochemistry; Biophysics; Molecular biology; Antigen-antibody reactions; Thermodynamics; Immunoglobulins; Chemistry; Analytic--Technique
Antibody fragments are attractive alternatives to conventional antibodies for both therapeutic and diagnostic applications. One example, single-domain VHH antibodies, which are derived from camelid heavy chain-only antibodies, display comparable binding affinity to conventional antibodies despite the significantly reduced binding interface. Here, an anti-RNase A VHH single-domain antibody and its antigen, RNase A, are used as a model antibody-antigen system for the three studies described in this dissertation.
Jakkaraju, Sriram, "The thermodynamic dissection and design of high-affinity antibodies" (2015). Graduate Research Theses & Dissertations. 6347.
Northern Illinois University
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