Frampton, Elon W.
M.S. (Master of Science)
Department of Biological Sciences
DNA; Escherichia coli; Enzymes; Biodegradation
In this investigation it was shown that the stage 3 products of the DNA degradation, initiated by colicin E2, in Escherichia coli K12 strains was caused by exonuclease 5 or an "exonuclease 5-like" enzyme. This was accomplished by using several recombination deficient mutants and looking at the pattern of DNA degradation by checking for TCA acid-insoluble radioactivity. One of the recombination deficient mutants further proved that endonuclease 1 was not the responsible enzyme. It was also seen that growth and division of the sensitive strains was necessary for colicin E2 to exert its biochemical effects. The immunity protein was removed from the complexed E2 protein by conventional methods and checked for its pattern of DNA degradation. Results obtained show that when the immunity protein is removed, there is an increased initial degradation of the DNA but the total degradation equaled the complexed E2 protein, in time, if added at the same multiplicities .
Ekenberg, Steven, "The role of exonucleases 5 in colicin E2-induced DNA degradation" (1982). Graduate Research Theses & Dissertations. 6245.
v, 48 pages
Northern Illinois University
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