Publication Date

1990

Document Type

Dissertation/Thesis

First Advisor

Hampel, Arnold E.

Degree Name

M.S. (Master of Science)

Department

Department of Biological Sciences

LCSH

Plant protoplasts||Catalytic RNA||Carrots||Autolysis

Abstract

Over the past eight years, several examples of catalytic RNAs have been identified and shown to cleave. In this study, a self-cleaving RNA cassette, derived from the "hairpin" catalytic RNA, will be used to test for in vivo autolysis of this RNA in carrot protoplasts. Plasmids for use as gene expression vectors in plants were constructed. These plasmids contained either the chloramphenicol acetyl transferase (CAT) gene or the hairpin cassette at the 3’ end of the CAT gene. Upon electroporation of these vectors into carrot protoplasts, cleavage of the hairpin RNA cassette could not be identified by northern analysis. An alternate system for this detection is considered, which involves SI nuclease analysis of plants stably transformed with the hairpin cassette.

Comments

Includes bibliographical references (pages 50-52)

Extent

v, 52 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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