Publication Date

1993

Document Type

Dissertation/Thesis

Degree Name

M.S. (Master of Science)

Department

Department of Biological Sciences

LCSH

Catalytic RNA||Genetic regulation||Gene expression

Abstract

Catalytic RNA is being used as a new tool to study the down regulation of gene expression. Our objective was to develop a model system to test the ability of the "hairpin" ribozyme to cleave target message RNA in vivo. A synthetic hairpin ribozyme was designed to cleave the maize alcohol dehydrogenase 1 (Adh1) message. Adh1 was chosen because activity of the protein is easily assayable and a selection system for stable transformants is available. The synthetic ribozyme (RADH) was designed to target native Adh1 mRNA at nucleotide position 3093 containing the requisite GUC cleavage site. Two pUC19 based vectors were engineered for in vivo expression of RADH at high levels in maize protoplasts. Transient and stable expression assays were performed to evaluate cleavage of the Adh1 mRNA transcript.

Comments

Includes bibliographical references (pages [58]-61)

Extent

vi, 63 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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