M.S. (Master of Science)
Department of Human and Family Resources
Improved glucose tolerance with rapid successive intravenous or oral glucose loads is known as the Staub- Traugott effect. To better understand this facilitated glucose disposal effect, glucose utilization (lipo- genesis) by epididymal and perirenal adipose tissues of normal, untreated diabetic, and insulin-treated diabetic rats was studied. Male Sprague-Dawley rats weighing 350-400 g in the post-absorptive state were injected 1.75 g/kg glucose in a 25% solution into the dorsal penal vein at 60-minute intervals under pentobarbitol. Along with the carrier glucose, 6 [lCi/100 g rat weight of U-l^c-D-glucose was given as either the first, second, or third challenge. Two hours after the labeled glucose, the animals were sacrificed and the epididymal and perirenal adipose tissues were removed and frozen. Tissues were extracted for lipid and the % recovered in each tissue was calculated. In the normal rats, mean % recovered from epididymal and perirenal tissues after the first and third load was significantly lower than the second load (P < 0.05). There was no significant difference between the % 14c recovered from the perirenal tissues of the untreated diabetic rats after the first and second load and also between the first and the third load, but the second load was significantly higher than the third load (P < 0.05). In the insulin-treated diabetic rats, the mean 14C recovered after the third load from both perirenal and epididymal adipose tissues was significantly higher than the mean % 14c recovered from the first load (P < 0.05). In all three groups of rats, the mean % 14C recovered from the epididymal and perirenal fat tissues did not vary. The insulin-treated diabetic rats showed increased lipogenesis with priming glucose loads, demonstrating the facilitatory glucose disposal phenomenon or the Staub-Traugott effect.
Varadarajan, Mahalakshmi, "The effect of priming glucose load(s) on lipogenesis in normal, untreated diabetic, and insulin-treated diabetic rats" (1989). Graduate Research Theses & Dissertations. 5547.
viii, 95 pages
Northern Illinois University
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