Lynch, Darrel L.
M.S. (Master of Science)
Department of Biological Sciences
This investigation was designed to determine the various metabolic pathways in Aotinoplanes philippinensis ATCC 11242 operating in the dissimilation of three different carbon sources; D-glucose, potassium gluconate (K- gluconate) and D-glucose supplemented with Na-acetate. Aotinoplanes philippinensis was grown in a chemically defined medium using D-glucose, K-gluconate and D-glueose- acetate. Spectrophotometric techniques were employed to assay for the enzymes of the Embden-Meyerhoff-Parnas pathway, the hexose monophosphate pathway, the Entner- Doudoroff pathway and the glyoxylate cycle. Enzymatic studies on the cell-free extracts of glucose-grown and glucose + acetate grown cells showed the presence of glucokinase, glucose-6-phosphate dehydrogenase, 6- phosphogluconate dehydrogenase, fructose diphosphate aldolase and isocitric dehydrogenase. Phosphoglucose isomerase is also known to be present in significant amounts in this organism. Gluconokinase and 2-keto-3- deoxy-6-phosphogluconate aldolase were found to be absent, unless the cells were adapted to grow on gluconate Malate synthase activity was detected in significant amounts only in glucose + acetate grown cells. On the basis of these results and earlier investigations made in this laboratory, it was concluded that the major route of glucose dissimilation in A. philippinensis is via the glycolytic pathway with a concomitant participation of hexose monophosphate pathway playing a minor role. The Entner-Doudoroff pathway is the major route of gluconate dissimilation in this microorganism with the pentose phosphate cycle playing a minor role by supplying intermediates for certain biosynthetic processes. The Glyoxylate cycle appears to be operative in A. philippinensis when acetate is present in the medium.
Abid, Syed Hasan, "The dissimilation of D-glucose and D-gluconic acid in Actinoplanes philippinensis" (1970). Graduate Research Theses & Dissertations. 5454.
viii, 88 pages
Northern Illinois University
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