Erman, James E.
M.S. (Master of Science)
Department of Chemistry
Imidazole; Chemical bonds; Hydrogen-ion concentration; Myoglobin
4-Nitroimidazole binding to horse heart metmyoglobin was studied as a function of pH and ionic strength. The pKa of 4- nitroimidazole was also studied as a function of ionic strength. It appears that two ionizable groups on the protein affect the observed association rate constant within the pH range of 5 to 11. The alkaline group, which has a pKa of 8.9, is responsible for the acid-alkaline transition of the protein. The more acidic group has a pKa near 6. This group only appears to influence the binding of 4-nitroimidazole at high ionic strengths. The ionic strength dependence of the rate constants does not indicate whether the neutral or anionic form of the ligand is preferentially bound to metmyoglobin. It is concluded that both forms of the ligand may contribute to the observed association rate constant.
Taylor, Kevin C., "The binding of 4-nitroimidazole to metmyoglobin : ionic strength and pH dependence of the association rate constant" (1981). Graduate Research Theses & Dissertations. 5342.
vi, 60 pages
Northern Illinois University
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