Publication Date

1996

Document Type

Dissertation/Thesis

First Advisor

Mitchell, John L. A.

Degree Name

M.S. (Master of Science)

Department

Department of Biological Sciences

LCSH

Cellular control mechanisms||Polyamines in the body||Nucleotide sequence||Ornithine decarboxylase

Abstract

Ornithine Decarboxylase (ODC) is a rate limiting enzyme in the synthesis of the polyamines whose role in the cell includes regulation of DNA replication, transcription and a variety of crucial growth related functions which are still not fully understood. The ODC gene is classified as an oncogene due to its overproduction in most cancerous growth. The DH[sub 23]b cells were derived from HTC rat hepatoma cells using the irreversible inhibitor of ornithine decarboxylase, diflouromethyl ornithine. DH[sub 23]b cells have a very high level of stable, active ODC, half-life of 4-6 hours, in contrast to the parental HTC ODC, which is highly labile, with a half-life of less than 20 minutes. Sequencing was done using the Sanger-Dideoxy method revealing a single base mutation in the DH[sub 23]b ODC gene but not the HTC ODC gene, resulting in the change of a cysteine to a tryptophan at amino acid position 441.

Comments

Includes bibliographical references (pages [42]-47)

Extent

iv, 47 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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