Author

Richard Tritz

Publication Date

1985

Document Type

Dissertation/Thesis

First Advisor

Hampel, Arnold E.

Degree Name

M.S. (Master of Science)

Department

Department of Biological Sciences

LCSH

Monoclonal antibodies||Enzymes||Ribonucleic acid

Abstract

Leucyl tRNA synthetase (LeuRS) has been purified several hundred-fold from Chinese hamster liver. The purification scheme was as follows: differential centrifugation, Alumina C gamma adsorption, DE-52 anion exchange column chromatography, and finally IGGF cation exchange column chromatography. The partially purified LueRS fraction obtained above was used as antigen in the production of monoclonal antibodies. Two immunization procedures were used. The in vivo procedure involved biweekly intraperitoneal injections of antigen in complete Freund's adjuvant. The in vitro procedure was done in 25 cm² culture flasks and the medium contained a synthetic peptidyl adjuvant. Using either procedure, the relative purity of the LeuRS fraction was important in the efficiency of specific anti- LeuRS clones produced.

Comments

Bibliography: pages 39-44.

Extent

vi, 44 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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