Conway, Thomas P.
M.S. (Master of Science)
Department of Biological Sciences
The major histocompatibility gene complex (MHC) produces a group of 45,000 dalton glycoproteins, the class I antigens, which are involved in the regulation of the immune response and in the rejection of foreign tissue and tumor grafts. Class I antigens are expressed on the membranes of most nucleated cells. β₂microglobulin, a 12,000 dalton polypeptide, is noncovalently bound to the class I antigens and is also found in unassociated form as a trace protein in the body fluids. To measure and compare the rates of production, distribution and catabolism of β₂microglobulin in normal and transformed cell lines it is necessary to distinguish between secreted free β₂microglobulin and histocompatibility antigen bound β₂microglobulin. To accomplish this, six monoclonal antibodies were produced: four which bind to antigenic determinants exposed in both bound and free β₂microglobulin for measurement of total β₂microglobulin production, and two which bind to a conformational antigenic determinant formed by association with the MHC complex for measurement of MHC bound β₂microglobulin only.
Gerity, Elizabeth, "Production of monoclonal antibodies to β₂microglobulin" (1983). Graduate Research Theses & Dissertations. 4531.
vii, 67 pages
Northern Illinois University
Rights Statement 2
NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.