Hampel, Arnold E.||Hanzely, Laszlo
M.S. (Master of Science)
Department of Biological Sciences
Regeneration (Biology); Corn; Sorghum
Two monocotyledonous angiosperm species were regenerated from established calli In order to produce transformed plants. Explant sources of Zea mays (A188, Black Mexican Sweet, and A188/BMS) consisted of complete embryos and scutellum-less embryos. Explant sources of Sorghum bicolor (Texas Blackhull Kafir) consisted of complete embryos, scutellum-less embryos, embryo-less scutellum, germinating mature seed, and seedling segments. Florets of S^ bicolor (Milo/tunis) were also cultured. Excision of smaller S. bicolor complete embryo, scutellum-less embryo, and embryo-less scutellum required the developmentof microexcision techniques to allow younger immature embryos to be successfully excised, resulting in more successful culturing. A single or double incision followed by precise organ removal resulted in less contamination of initiating cultures compared to previously described methods. The explant tissues of Z. mays were cultured upon a modified Murashige and Skoog (MS) medium containing 1.0, 1.5, or 2.0 mg/1 of 2,4-D. All S. bicolor explant tissues were cultured on a modified MS medium containing zero, 1.0, 1.5, or 2.0 mg/1 of 2,4-D. Calli were obtained from all tissue sources in response to the varying concentrations of 2,4-D, suggesting that all undifferentiated tissues attempt to react alike when influenced by similar hormonal imbalances. Callus initiation from the various explant sources of both species was of the highest quality on media containing 2.0 mg/1 2,4-D, except the calli derived from viviparous florets of S. bicolor which favored 1.0 mg/1 2,4-D. Maintenance of calli derived from the various explant sources of both species was possible on media containing 1.0, 1.5 or 2.0 mg/1 2,4-D. The calli derived from both species had more regional differentiation on media containing 1.0 mg/1 2,4-D. No improvements in the embryogenic behavior of the S. bicolor calli were observed following cold treatment (vernalization), or inversion of Petri dishes containing estalished calli (geotropic effect). Plants of Z. mays were regenerated only from established calli derived from complete embryos. Plants of S. bicolor were regenerated only from established calli derived from scutellum-less embryos.
Kilfoy, E. J., "Plant regeneration from established calli derived from isolated intact embryos of Zea mays and isolated scutellum-less embryos of Sorghum bicolor" (1986). Graduate Research Theses & Dissertations. 4410.
vi, 78 pages
Northern Illinois University
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